Journal: Neurobiology of disease

Article Title: DJ-1 ameliorates ischemic cell death in vitro possibly via mitochondrial pathway

doi: 10.1016/j.nbd.2013.09.007

Figure Lengend Snippet: DJ-1 selectively translocates to the electrochemically active mitochondria. For visualization of the mitochondrial membrane potential, cells were incubated with 100 nM MitoTracker (red) for 30 min before cell fixation (as described in the Materials and methods), and then immunostained for anti-DJ-1- (green) and anti-ATP synthase (mitochondrial) β-chain (blue). Depolarized (yellow arrows) and electrochemically active (white arrows) mitochondria are shown to indicate mitochondria that are impermeable and permeable to the dye MitoTracker, respectively. DJ-1 co-localized with electrochemically active mitochondria, but not with depolarized mitochondria. Scale bars = 5 μm.

Article Snippet: After blocking reaction with 5% normal goat serum (Vector), the cells were incubated overnight at 4 °C with rabbit monoclonal anti-DJ-1 (1:100; Abcam, ab76008) and mouse monoclonal anti-ATP synthase (mitochondrial) β-chain (1:200; Cell Signaling Technologies, 05-709) with 5% normal goat serum.

Techniques: Membrane, Incubation